Action potential alterations induced by single F11 neuronal cell loading

Several research programmes have demonstrated how Transcranial Ultrasound Stimulation (TUS) can non-invasively and reversibly mechanically perturb neuronal functions. However, the mechanisms through which such reversible a priori non-damaging behaviour be observed remain largely unknown. While several TUS protocols motor behavioural alterations in vivo models, vitro studies scarce. In particular, an experimental framework able to load individual neuron controlled manner simultaneously measure generation evolution of action potentials before, during after load, while allowing for direct microscopy, has not been successfully proposed. To this end, we herein present multiphysics setup combining nanoindentation patch clamp systems, assembled inverted microscope simultaneous bright-field or fluorescence imaging. We evaluate potential platform with set experiments single dorsal root ganglion-derived cell bodies are compressed their spontaneous activity is recorded. show that these transient quasi-static mechanical loads affect amplitude rate change potentials, smaller slower upon indentation, irreversibly altering other features. The ability image, electrically manipulate record cells perturbed environment makes system particularly suitable studying brain at level.